Duke RNAi Screening Facility

About the RNAi Facility

The Center for RNA Biology and the IGSP have established an RNAi Facility for the application of RNA interference (RNAi) technologies for mammalian functional genomics. The primary mission of the RNAi Facility is to develop and deploy RNAi technologies to support the research programs of investigators at Duke and beyond. Through investments in RNAi and complementary technologies, we have assembled state-of-the-art functional genomics infrastructure, providing researchers access to genome-wide RNAi reagents and the infrastructure necessary to conduct large-scale loss-of-function studies in mammalian cells.

• RNAi expertise: The RNAi Facility serves as the focal point of RNAi technology development and expertise for the Duke University and Medical Center research community, and will work to advance and apply gene silencing technologies to a broad range of research programs.
• RNAi reagents: The availability of RNAi libraries targeting entire genomes or subsets provides the opportunity to conduct loss-of-function genetic screens and selections in mammalian cells. The RNAi Facility has acquired several human whole-genome RNAi collections. The Qiagen Human whole-genome siRNA library v1.0, the TRC1 lentiviral shRNA library and an MSCV-based retroviral shRNA library The siRNA library may be screened in arrayed format, subsets of the TRC1 library may be screened in arrayed or pooled format and the MSCV retroviral library is available only in pooled format.
• Genome-wide RNAi studies: The RNAi Facility has developed the infrastructure necessary to conduct High-throughput RNAi based screening on a genome-wide scale. In addition, we have the ability to perform High-content cell-based imaging and analysis. The Facility will work with researchers to develop, evaluate, and implement cell-based screening and/or selection studies

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